Serena Williams of the United States acknowledges the audience after losing to Australia's Ajla Tomljanovic in the third round of the U.S. Open tennis championships on Friday, September 2, 2022, in New York.

Serena Williams of the United States returns a shot against Australia's Ajla Tomljanovic during the third round of the US Open tennis championships on Friday, September 2, 2022, in New York.

A sentimental Serena Williams lost in the third round of the US Open to Ajla Tomljanovic, which could have been her final singles match.

Williams, who turns 41 this month, said before the competition that she was planning to retire from tennis.

She did not say when it would be, but it would be after the US Open, where she won six of her 23 Grand Slam singles titles.

On day five of the 2022 U.S. Open tennis competition at the USTA Billie Jean King Tennis Center,

Serena Williams of the United States hits to Ajla Tomljanovic of Australia.

Serena, despite having tears in her eyes and a wide range of emotions, managed to smile while speaking with a tournament official after the match.

On day five of the 2022 U.S. Open tennis championship at the USTA Billie Jean King Tennis Center,

 Serena Williams of the United States reacts during a match against Ajla Tomljanovic of Australia.